Journal: Frontiers in Microbiology

Article Title: Identification of Antimicrobial Compounds in Two Streptomyces sp. Strains Isolated From Beehives

doi: 10.3389/fmicb.2022.742168

Figure Lengend Snippet: Metabolic characterization by UPLC and LC-MS analyses of cell extracts from cultures of Streptomyces griseoaurantiacus AD2 (A) and Streptomyces albus AN1 (B) strains. (A) S. griseoaurantiacus AD2 extracts were generated from 96 h-cultures in R5A (green) or SM17 (orange), and assayed against M. luteus . The graphs below the UPLC chromatograms show the UV spectrum of the metabolites that might display antimicrobial activity (see peaks named as “u” and “m”). The corresponding molecular weights were calculated by LC-MS analyses (and also by dereplication analyses), resulting in the identification of the metabolites undecylprodigiosin and manumycin A. (B) S. albus AN1 extracts were generated from 96 h-cultures in R5A (green), SM17 (orange) and Bennet (red), and assayed against C. albicans . The peaks selected from the AN1 cultures possess an UV spectrum that match that of the candicidins and antimycins.

Article Snippet: The two isolates were assessed for growth inhibition against different types of indicator microorganisms, including Micrococcus luteus ATCC 23262, Bacillus cereus ATCC 14579, Enterococus faecalis ATCC 33186, Staphylococcus aureus BAA-1747, Streptomyces coelicolor M145, Escherichia coli ESS, Pseudomonas aeruginosa ATCC 10145, Salmonella enterica subsp. enterica serovar Typhimurium ATCC14028, Candida albicans ATCC 32077, and Saccharomyces cerevisiae VL6-48.

Techniques: Liquid Chromatography with Mass Spectroscopy, Generated, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Organic-Inorganic Biocompatible Coatings for Temporary and Permanent Metal Implants

doi: 10.3390/ijms252111623

Figure Lengend Snippet: Permanent Metal Implants with Organic Coatings.

Article Snippet: Ti-15Mo , Ca(H 2 PO 2 ) 2 ; voltage 300 V, current density 100 mA·cm −2 t = 5 min , surface roughness R z = 1.5 ± 0.3 μm, R a = 0.5 ± 0.09 μm , PLGA (M n 19,000), PLGA/DOX , PLGA: R z = 2.3 ± 0.9 μm, R a = 1.2 ± 0.2 μm; PLGA/DOX: R z = 4.9 ± 0.9 μm, R a = 1.2 ± 0.1 μm , Cell viability: MG-63, after 7 days, the highest increase in the cell number was observed for PEO/PLGA–21.17%. For PEO/PLGA/DOX the proliferation decreased to 10.30%, but it was higher than in the case of Ti-15Mo and Ti-15Mo/PEO. Antibacterial efficacy: S. aureus (ATCC 25923), S. epidermidis (ATCC 700256), the amount of loaded doxycycline was sufficient to inhibit bacterial growth. , [ ] .

Techniques: Modification, Bacteria, Concentration Assay, Inhibition, Polymer, Comparison, Activity Assay, In Vivo, Infection, Incubation, In Vitro, Irradiation, Pore Size